DEAE Sephadex A-25

DEAE Sepharose Fast Flow deae The pKa of DEAE is approximately If the resin is equilibrated with buffer at pH , what will be the overall charge of the stationary phase? Positive deae DEAE Sephadex™ A-25 and DEAE Sephadex A-50 are weak anion exchangers The ion exchange group is diethylaminoethyl which remains charged and maintains

deae As a starting point for developing a separation, we recommend using the Bio-Scale DEAE 2 column with a simple gradient profile over 48 ml Protocol: Use a flow